| Customization: | Available |
|---|---|
| Classification: | Imaging Diagnostic Equipment |
| Type: | Cellulose Acetate Membrane |
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Cellulose acetate is processed into film, and the commonly used specifications are 2*8cm, 8*12cm and any specifications that do not exceed the processing mold, which is used in scientific research and other fields. Because cellulose acetate has a very small adsorption effect on proteins and the hydrophilicity of film is small, it is often used in serum protein electrophoresis experiments, and the operation is simple and quick. The serum protein electrophoresis experiment is introduced as follows:
(I) Main instruments
Electrophoresis machine, petri dish, filter paper, tweezers, glass slide or sample applicator
(II) Liquid preparation
1. PH 8.6 barbital buffer solution: (ionic strength 0.06)
Barbital sodium 12.76g, barbital 1.66g, add distilled water to 1000ml to fully dissolve
2. Dyeing solution
Amido black 10B 0.25g, add methanol 50mL, glacial acetic acid 10mL, distilled water 40mL
3. Rinsing solution
45mL ethanol, 5mL glacial acetic acid, 50mL distilled water
4. Leachate
0.4mol/L sodium hydroxide
5. Transparent solution
25ml glacial acetic acid, 75ml ethanol
(II) Operation method
1. Dip the film of appropriate specification into the barbital buffer solution with the glossy side downward, take it out after the film is completely soaked without white spots, absorb the excess buffer solution with filter paper, and then spot the sample at a distance of 1.5cm from one end of the matte side of the film. After the sample is immersed in the film,place the film on the filter paper bridge of the electrophoresis tank holder with spotting side downward and the spotting end against the negative electrode.
2. Cover the electrophoresis tank. The voltage is 90-120V, the current is 0.4-0.6mA/cm, and the time is 45-60 minutes. Turn off the power after the zone is unfolded.
3. Immerse the film into the dyeing solution, take it out after 5-10 minutes and transfer it to the rinsing solution to decolor several times. The rinsing solution can be divided into one rinse and two rinses for multiple containers, and the color is decolorized until the background is colorless.
4. Perform quantitative analysis or transparent treatment on the decolorized film.
(IV) Precautions
When the film is immersed, the glossy side should be facing down to absorb the buffer solution more evenly. If there are still spots after a long-time immersion, it should be discarded. Be sure to cover the electrophoresis tank during electrophoresis. The electrophoresis speed is inversely proportional to the buffer concentration or ionic strength within the effective range.
FAQ:
Q1: Why Choose NANBEI ?
(1).Professional manufacturer with more than 13 years experience
(2).Exported to more than 97% Countries
(3).Turnkey Solution is no problem
Q2:OEM,ODM acceptable or not?
Absolutely Yes
Q3:What's kind of Payment terms for customer choosing?
T/T ,Western Union, Money Gram , Credit Card, Paypal , L/C ...
Q4:Can you visit your factory online?
Absolutely no problem
Q5:Can online video inspection before shipment?
Absolutely no problem
Q6: what's the MOQ ? Sample order is OK?
MOQ:1 set, sample order is no problem
Q7:What's kind of shipment for customer choosing?
Usually ship by sea, by air, by international express .
We can also provide reasonable solutions according to your transportation requirements
Q8:How to ensure product quality and after-sales service?
We have CE, ISO quality certificate, and SGS authentication.
After-sale service:
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